TABLE 1.
Primers used in this study
| No. | Name | Sequencea |
|---|---|---|
| 1 | YscP1-15 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGAACCTGAGTATCAAGGTAC |
| 2 | YscP1-15 Nde-Kpn 2 | CTTGATACTCAGGTTCTAATGGGGAACGAGTGGTGATTTTATTCA |
| 3 | YscP1-14 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGAACCTGAGTATGGTAC |
| 4 | YscP1-14 Nde-Kpn 2 | CATACTCAGGTTCTAATGGGGAACGAGTGGTGATTTTATTCA |
| 5 | YscP1-13 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGAACCTGAGGGTAC |
| 6 | YscP1-13 Nde-Kpn 2 | CCTCAGGTTCTAATGGGGAACGAGTGGTGATTTTATTCA |
| 7 | YscP1-12 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGAACCTGGTAC |
| 8 | YscP1-12 Nde-Kpn 2 | CAGGTTCTAATGGGGAACGAGTGGTGATTTTATTCA |
| 9 | YscP1-11 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGAAGGTAC |
| 10 | YscP1-11 Nde-Kpn 2 | CTTCTAATGGGGAACGAGTGGTGATTTTATTCA |
| 11 | YscP1-10 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCATTAGGTAC |
| 12 | YscP1-10 Nde-Kpn 2 | CTAATGGGGAACGAGTGGTGATTTTATTCA |
| 13 | YscP1-9 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCCCAGGTAC |
| 14 | YscP1-9 Nde-Kpn 2 | CTGGGGAACGAGTGGTGATTTTATTCA |
| 15 | YscP1-8 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTTCCGGTAC |
| 16 | YscP1-8 Nde-Kpn 2 | CGGAACGAGTGGTGATTTTATTCA |
| 17 | YscP1-7 Nde-Kpn 1 | TATGAATAAAATCACCACTCGTGGTAC |
| 18 | YscP1-7 Nde-Kpn 2 | CACGAGTGGTGATTTTATTCA |
| 19 | YscP1-6 Nde-Kpn 1 | TATGAATAAAATCACCACTGGTAC |
| 20 | YscP1-6 Nde-Kpn 2 | CAGTGGTGATTTTATTCA |
| 21 | YscP15 mut NdeI-KpnI F | TATGAACAAGATAACAACGCGGAGCCCCCTGGAGCCGGAATACCAGGGTAC |
| 22 | YscP15 mut NdeI-KpnI R | CCTGGTATTCCGGCTCCAGGGGGCTCCGCGTTGTTATCTTGTTCA |
| 23 | YscP 15 + 1 NdeI-KpnI F | TATGAAATATAATCACCACTCGTTCCCCATTAGAACCTGTGTATCAGGTAC |
| 24 | YscP 15 + 1 NdeI-KpnI R | CTGATACACAGGTTCTAATGGGGAACGAGTGGTGATTATATTTCA |
| 25 | 3′YscP300 BglII | AAAGATCTGGTATCTTTGCTGCTATCAACGCCAT |
| 26 | 3′YscP200 BglII | AAAGATCTGACAGACCACACAATAACCTCTGCG |
| 27 | 3′YscP100 BglII | AAAGATCTGTTTAAATCATGATTATTCTGATGGT |
| 28 | 3′YscP25 BglII | AAAGATCTTTGCAAATCATGATGCAGCTTCCCC |
| 29 | 5′YscP-NdeI | AACAACAACATATGAATAAAATCACCACTCGTTCCCCATTAG |
| 30 | NdeI YscP Δ2-15 F | GGGAATTCCATATGCCTCTGGGGAAGCTGCATCATGATTTGCAA |
| 31 | NdeI YscP Δ2-50 F | GGGAATTCCATATGAGACCTGTACGTCCGCATGACCTTGGC |
| 32 | NdeI YscP Δ2-100 F | GGGAATTCCATATGAACTTATCTCCGCTTGCCGAAGGTGTTACC |
| 33 | NdeI YscP Δ2-150 F | GGGAATTCCATATGAGTGAATCCCCCTGCGAGCCGTCTGGACAT |
| 34 | KpnI YscP R (no stop) | CGGGGTACCTTCTTCAGCCTCCCACTCCTCATAGACGTGGCG |
a
Boldface indicates restriction sites.