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Binding of PA0964 at the pqsR promoter region, shown by electrophoretic mobility shift assay. PCR products containing the PpqsR and PlasR promoter regions and PPA1243 were added to the reaction mixtures at 50 ng each. PA0964 protein was added to the reaction mixtures in lanes B to D at 320 ng, 640 ng, and 960 ng, respectively. No protein was added in lane A. DNA of the PpqsR region was specifically bound by PmpR (PpqsR-PmpR).
