FIG. 7.

GC-MS analysis of sesquiterpenoids produced by fusion-type terpene synthase NP2 from N. punctiforme. Culture supernatant of E. coli cells expressing fusion-type sesquiterpene synthase NP2 for N. punctiforme was analyzed for the accumulation of sesquiterpene products. One major peak (peak 1) with an m/z of 222 and a typical sesquiterpene alcohol fragmentation pattern was identified as (E,E)-germacradienol. Two other peaks were identified as germacrene D (peak 2) and β-elemene (peak 3; Cope rearrangement product of germacrene A). Mass spectra for peaks 1 and 2 are shown.