TABLE 2.
Activities of reporter fusions to the tynA and feaB promoters in nsrR and feaR mutantsa
| Genotype | β-Galactosidase activity (Miller Units)b
|
|||||
|---|---|---|---|---|---|---|
| Glycerol
|
Phenylethylamine
|
Nitrotyramine
|
||||
| tynA-lacZ | feaB-lacZ | tynA-lacZ | feaB-lacZ | tynA-lacZ | feaB-lacZ | |
| Wild type | ND | 147 (9) | 849 (30) | 1,055 (51) | 333 (14) | 420 (37) |
| ΔnsrR | ND | 182 (10) | 1024 (81) | 1,570 (61) | 435 (5) | 487 (15) |
| feaR::kan | ND | 153 (15) | NDc | 87 (1) | NG | NG |
| ΔnsrR feaR::kan | ND | 195 (3) | ND | 104 (7) | NG | NG |
a
Cultures were grown at 30°C in defined medium containing 5 mM phenylethylamine or 40 mM glycerol as the carbon source or containing 11.1 mM glucose as the carbon source with 1.31 mM nitrotyramine as the nitrogen source.
b
Numbers in parentheses are 1 standard deviation. Units are as defined by Miller (25). ND, not detectable (<2 units μmol/min/mg protein). NG, no growth.
c
Growth of the feaR mutant on phenylethylamine was severely impaired.