TABLE 4.
Growth substrate | O2 uptake (nmol min−1 mg protein−1)b
|
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---|---|---|---|---|
MG1655
|
MG1655 ΔnsrR
|
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Phenylethyl- amine | 3-Nitroty- ramine | Phenylethyl- amine | 3-Nitroty- ramine | |
Phenylethylamine | 66 (5) | 41 (3) | 73 (11) | 44 (7) |
Glycerol | ND | ND | ND | 3 (1) |
3-Nitrotyramine | 6 (3) | 4 (2) | 7 (2) | 4 (1) |
Phenylethylamine- and 3-nitrotyramine-dependent O2 uptake in extracts of cells grown on phenylethylamine or glycerol (as the carbon source) or on 3-nitrotyramine (as the nitrogen source). Cultures were grown at 30°C in defined medium containing 5 mM phenylethylamine or 40 mM glycerol as the carbon source or containing 11.1 mM glucose as the carbon source with 2.56 mM nitrotyramine as the nitrogen source.
Numbers in parentheses are 1 standard deviation (SD). ND, not detectable (<2 nmol min−1 mg protein−1).