TABLE 5.

Phenylethylamine- and 3-nitrotyramine-dependent NAD⁺ reduction^a

Growth substrate	NAD+ reduction (nmol min−1 mg protein−1)b
	MG1655		MG1655 ΔnsrR
	Phenylethyl- amine	3-Nitroty- ramine	Phenylethyl- amine	3-Nitroty- ramine
Phenylethylamine	38 (2.3)	18 (1.6)	35 (2.8)	15 (2.2)
Glycerol	ND	2.4 (1.0)	1.0 (0.7)	1.3 (0.3)
3-Nitrotyramine	54 (2.5)	20 (6.4)	42 (4.4)	14 (4.0)

^aData show phenylethylamine- and 3-nitrotyramine-dependent NAD⁺ reduction in extracts of cells grown on phenylethylamine or glycerol (as the carbon source) or on nitrotyramine (as the nitrogen source). Cultures were grown at 30°C in defined medium containing 5 mM phenylethylamine or 40 mM glycerol as the carbon source or containing 11.1 mM glucose as the carbon source with 1.3 mM nitrotyramine as the nitrogen source.

^bNumbers in parentheses are 1 standard deviation (SD). ND, not detectable (<1 nmol min⁻¹ mg protein⁻¹).