TABLE 5.
Growth substrate | NAD+ reduction (nmol min−1 mg protein−1)b
|
|||
---|---|---|---|---|
MG1655
|
MG1655 ΔnsrR
|
|||
Phenylethyl- amine | 3-Nitroty- ramine | Phenylethyl- amine | 3-Nitroty- ramine | |
Phenylethylamine | 38 (2.3) | 18 (1.6) | 35 (2.8) | 15 (2.2) |
Glycerol | ND | 2.4 (1.0) | 1.0 (0.7) | 1.3 (0.3) |
3-Nitrotyramine | 54 (2.5) | 20 (6.4) | 42 (4.4) | 14 (4.0) |
Data show phenylethylamine- and 3-nitrotyramine-dependent NAD+ reduction in extracts of cells grown on phenylethylamine or glycerol (as the carbon source) or on nitrotyramine (as the nitrogen source). Cultures were grown at 30°C in defined medium containing 5 mM phenylethylamine or 40 mM glycerol as the carbon source or containing 11.1 mM glucose as the carbon source with 1.3 mM nitrotyramine as the nitrogen source.
Numbers in parentheses are 1 standard deviation (SD). ND, not detectable (<1 nmol min−1 mg protein−1).