Figure 6.

Inhibition of Tkv-induced oligomerization of Mad by Dad. (A) The effect of Dad on hetero-oligomerization of Mad with Smad4 was examined. Cells were transfected with the indicated combinations of plasmids. The lysates were subjected to immunoprecipitation with anti-FLAG antibody followed by immunoblotting with anti-myc antibody. Expression levels of proteins were monitored by Western blotting. (B) Effect of Dad on homo-oligomerization of Mad was examined. Cells were transfected with the indicated combinations of plasmids including FLAG-tagged and myc-tagged Mad. The lysates were subjected to immunoprecipitation with anti-FLAG antibody followed by immunoblotting with anti-myc antibody. Expression levels of proteins were monitored by Western blotting.