FIG. 4.
Steady-state levels of GFP fusion proteins coexpressed with ZapA fusions. Total cell extracts from strains (CR208 to CR219 and CR373 to CR376, Table 1) coexpressing GFP-DivICflag3, GFP-FtsLBflag3, GFP-DivIBmyc3, or GFP-PBP 2Bmyc3 with ZapA-DivICflag3 (IC), ZapA-FtsLBflag3 (L), ZapA-DivIBmyc3 (IB), or ZapA-FtsB (/) as a negative control were analyzed by SDS-PAGE and immunoblotting with anti-GFP antibodies. The molecular masses of the standards are indicated to the right of the blot in kilodaltons. The “★” symbol indicates degradation products.