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. 2008 Jul 11;190(18):6048–6059. doi: 10.1128/JB.00462-08

FIG. 6.

FIG. 6.

Steady-state levels of DivICflag3 and FtsLBflag3 GFP or ZapA fusions coexpressed with DivICflag3, DivIBmyc3, PBP 2Bmyc3, or FtsLBflag3. (A) Total cell extracts from strains (CR198 and CR200 carrying the plasmids pBAD33, pCR52, pCR54, pCR60, or pCR63 [Table 1]), coexpressing GFP-DivICflag3 or GFP-FtsLBflag3, with DivICflag3 (IC), DivIBmyc3 (IB), PBP 2B myc3 (PBP), FtsLBflag3 (L) or carrying an empty plasmid (/) as a negative control were analyzed by SDS-PAGE and immunoblotting with anti-GFP antibodies. (B) Total cell extracts from strains JOE309 coexpressing ZapA-DivICflag3 (from pCR39) or ZapA-FtsLBflag3 (from pCR41) with DivICflag3 (IC) (from pCR52) or FtsLBflag3 (L) (from pCR63) or carrying an empty plasmid (/) as a negative control were analyzed by SDS-PAGE and immunoblotting with anti-Flag antibodies. Molecular masses of the standards are indicated to the right of the blot in kDa. The “★” symbol indicates degradation products.