TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or features | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 ΔlacU169 relA1 rpsL150 thi mot flb5301 deoC7 ptsF25 rbsR | Laboratory collection |
| JOE309 | MC4100 ara+ | 11 |
| KS272 | F− ΔlacX74 galE galK thi rpsL ΔphoA (ΔPvuII) | Laboratory collection |
| CR14 | JOE309 ΔftsB(Kanr)/pBAD42-ftsB | This study |
| JC60 | KS272 ftsI::TnphoAI137ΔIS50R(Kanr)/pBAD33-ftsI | Laboratory collection |
| JOE417 | JOE309 ftsQE14::kan/pJC10 | 10 |
| NB988 | JOE307 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | N. Buddelmeijer |
| CR166 | CR14/pCR31 | This study |
| CR168 | NB988/pCR34 | This study |
| CR198 | JOE309 Δ(λattL-lom)::bla lacIq pDSW204-gfp-divICflag3 | This study |
| CR200 | JOE309 Δ(λattL-lom)::bla lacIq pDSW204-gfp-ftsLBflag3 | This study |
| CR202 | JOE309 Δ(λattL-lom)::bla lacIq pDSW204-gfp-divIBmyc3 | This study |
| CR208 | CR198/pMDG1 | This study |
| CR209 | CR198/pCR39 | This study |
| CR210 | CR198/pCR40 | This study |
| CR211 | CR198/pCR41 | This study |
| CR212 | CR200/pMDG1 | This study |
| CR213 | CR200/pCR39 | This study |
| CR214 | CR200/pCR40 | This study |
| CR215 | CR200/pCR41 | This study |
| CR216 | CR202/pMDG1 | This study |
| CR217 | CR202/pCR39 | This study |
| CR218 | CR202/pCR40 | This study |
| CR219 | CR202/pCR41 | This study |
| CR251 | CR208 ftsQE14::kan/pJC10 | This study |
| CR253 | CR211 ftsQE14::kan/pJC10 | This study |
| CR257 | CR213 ftsQE14::kan/pJC10 | This study |
| CR275 | CR198/pNG162 | This study |
| CR278 | CR198/pCR44 | This study |
| CR279 | CR200/pNG162 | This study |
| CR280 | CR200/pCR42 | This study |
| CR315 | JOE417/pCR38 | This study |
| CR362 | JOE309 Δ(λattL-lom)::bla lacIq pDSW204-gfp-pbpBmyc3 | This study |
| CR368 | JC60/pCR67 | This study |
| CR373 | CR362/pMDG1 | This study |
| CR374 | CR362/pCR39 | This study |
| CR375 | CR362/pCR40 | This study |
| CR376 | CR362/pCR41 | This study |
| Plasmids | ||
| pBAD33 | Arabinose-regulated promoter, pACYC184 origin, Cmr | 28 |
| pBAD42 | Arabinose-regulated promoter, pSC101 origin, Specr | 28 |
| pDSW204 | IPTG-regulated promoter, pBR322 origin, Ampr | 47 |
| pDSW207 | pDSW204-gfp-MCS (fusion vector) | 47 |
| pBAD42-ftsB | pBAD42-ftsB | N. Buddelmeijer |
| pJC10 | pBAD33-ftsQ | 11 |
| pJMG197 | pBAD33-ftsL | 22 |
| pMDG1 | pNG162-zapA-Fcyto-ftsB | M. D. Gonzalez |
| pNG162 | IPTG regulated promoter (pDSW204), pSC101 origin, Specr | 24 |
| pCR23 | pBAD33-myc3 | This study |
| pCR25 | pDSW207-flag3 | This study |
| pCR27 | pDSW207-myc3 | This study |
| pCR31 | pDSW204-gfp-divICflag3 | This study |
| pCR34 | pDSW204-gfp-ftsLBflag3 | This study |
| pCR38 | pDSW204-gfp-divIBmyc3 | This study |
| pCR39 | pNG162-zapA-Fcyto-divICflag3 | This study |
| pCR40 | pNG162-zapA-Fcyto-divIBmyc3 | This study |
| pCR41 | pNG162-zapA-Fcyto-ftsLBflag3 | This study |
| pCR42 | pNG162-divICflag3 | This study |
| pCR44 | pNG162-ftsLBflag3 | This study |
| pCR67 | pDSW204-gfp-pbpBmyc3 | This study |
| pCR52 | pBAD33-divICflag3 | This study |
| pCR54 | pBAD33-divIBmyc3 | This study |
| pCR60 | pBAD33-pbpBmyc3 | This study |
| pCR63 | pBAD33-ftsLBflag3 | This study |
| pCR68 | pBAD33-pbpBmyc3-divICflag3 | This study |
aCmr, chloramphenicol resistance; Specr, spectinomycin resistance; Ampr, ampicillin resistance; Kanr, kanamycin resistance.