. 2008 Jul 11;190(18):6048–6059. doi: 10.1128/JB.00462-08

TABLE 2.

E. coli artificial septal targeting assays performed with the coexpression of three or four B. subtilis proteins^a

Third protein	ZapA fusions
	GFP-DivIC_flag3			GFP-FtsL_Bflag3			GFP-DivIB_myc3			GFP-PBP 2B_myc3
	IC	L_B	IB	IC	L_B	IB	IC	L_B	IB	IC	L_B	IB
Empty pBAD33 plasmid	-	+	-	+	-	-	-	-	-	-	-	+
DivIC_flag3	ND	+	-	+	-	-	-	-	-	-	-	+
FtsL_Bmyc3	-	+	-	+	ND	-	-	ND	-	-	-	+
DivIB_myc3	-	+	-	+	-	-	-	-	-	-	-	+
PBP2B_myc3	-	+	-	+	-	-	-	-	-	-	-	+
PBP2B_myc3 + DivIC_flag3	ND	ND	ND	+	ND	-	-	-	-	ND	ND	ND

Subcellular localization of DivIC_flag3, FtsL_Bflag3, DivIB_myc3, and PBP 2B_myc3 GFP fusions has been observed in strains coexpressing them with DivIC_flag3 (IC), FtsL_Bflag3 (L_B), or DivIB_myc3 (IB) ZapA fusions and with an empty pBAD33 plasmid or DivIC_flag3, FtsL_Bmyc3, DivIB_myc3, PBP 2B_myc3 or (PBP 2B_myc3+ DivIC_flag3) expressed from plasmid pBAD33. Strains (from CR209 to CR211, CR213 to CR215, CR217 to CR219, and CR374 to CR376; Table 1) transformed with pBAD33, pCR52, pCR54, pCR60, pCR63, or pCR68 (Table 1) were grown at 30°C, and tested proteins were induced for 1 h with 50 μM IPTG and 0.2% arabinose. Samples were then prepared for microscopy as described in Materials and Methods. +, Septal localization of the GFP fusion; -, diffuse GFP signal; ND, not determined.