TABLE 1.
Primers used in this study
| Primer | Linker | 5′-3′ Sequence |
|---|---|---|
| Xba_mRFP_Mega | XbaI | CAGTCTAGAATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGG |
| ChFP_rev | HindIII | GCAAGCTTTTACTTGTACAGCTCGTCCATG |
| omlA_for | EcoRI | GCGAATTCGCTATCGGCGAGCTCGAACAC |
| omlA_rev | XbaI | GCTCTAGAAGGAAACGAGCAACCGGCGAG |
| CX_ChFP_for1 | ATGGCCTCCTCCGAGGACGTCATC | |
| CD_ChFP_mut | TCTGGAAGGAAAGTCGCAACCGGCGAGTGCGGCAAGCCC | |
| 4370_for | EcoRI | GCGAATTCGGACGGAGCTCCGGACGAAG |
| 4370_rev | XbaI | GCTCTAGACTTGATCACCGCCTTGGCGG |
| 4370_rev2 | XbaI | GCTCTAGAGAACTCGTGGTCGGCGTTGTC |
| 4370_mut1 | GGCGCTTCGGCTTTCTTAGGAAACGAGCAGCCGGCGAGGGAG | |
| 4370_mut2 | CTCCCTCGCCGGCTGCTCGTTTCCTAAGAAAGCCGAAGCGCC | |
| 3262_for | EcoRI | GCGAATTCTCCTTCCTCAGCTGTATCTCTTG |
| 3262_rev | XbaI | GCTCTAGATGCGAATCGCAGCCGGAGAGAAC |
| FNS-F | GCACTCGCCGGTTGCTTCAACAGCGGCGCCTCCTCCGAGGACG | |
| FNS-R | CGTCCTCGGAGGAGGCGCCGCTGTTGAAGCAACCGGCGAGTGC | |
| KVE-F | GCACTCGCCGGTTGCAAGGTGGAGGGCGCCTCCTCCGAGGACG | |
| KVE-R | CGTCCTCGGAGGAGGCGCCCTCCACCTTGCAACCGGCGAGTGC | |
| DSQ-F | GCACTCGCCGGTTGCGACAGCCAGGGCGCCTCCTCCGAGGACG | |
| DSQ-R | CGTCCTCGGAGGAGGCGCCCTGGCTGTCGCAACCGGCGAGTGC | |
| GKS-F | GCACTCGCCGGTTGCGGCAAGTCGGGCGCCTCCTCCGAGGACG | |
| GKS-R | CGTCCTCGGAGGAGGCGCCCGACTTGCCGCAACCGGCGAGTGC | |
| GGC-F | GCACTCGCCGGTTGCGGCGGCGGCGGCGCCTCCTCCGAGGACG | |
| GGC-R | CGTCCTCGGAGGAGGCGCCGCCGCCGCCGCAACCGGCGAGTGC | |
| GNG-F | GCACTCGCCGGTTGCGGCAACGGCGGCGCCTCCTCCGAGGACG | |
| GNG-R | CGTCCTCGGAGGAGGCGCCGCCGTTGCCGCAACCGGCGAGTGC | |
| GDD-F | GCACTCGCCGGTTGCGGCGACGACGGCGCCTCCTCCGAGGACG | |
| GDD-R | CGTCCTCGGAGGAGGCGCCGTCGTCGCCGCAACCGGCGAGTGC | |
| AST-F | GCACTCGCCGGTTGCGCCAGCACCGGCGCCTCCTCCGAGGACG | |
| AST-R | CGTCCTCGGAGGAGGCGCCGGTGCTGGCGCAACCGGCGAGTGC | |
| QGS-F | GCACTCGCCGGTTGCCAGGGCAGCGGCGCCTCCTCCGAGGACG | |
| QGS-R | CGTCCTCGGAGGAGGCGCCGCTGCCCTGGCAACCGGCGAGTGC | |
| SSQ-F | GCACTCGCCGGTTGCAGCAGCCAGGGCGCCTCCTCCGAGGACG | |
| SSQ-R | CGTCCTCGGAGGAGGCGCCCTGGCTGCTGCAACCGGCGAGTGC |