FIG. 2.

Redox absorption spectra of the SoxAX-CT1020 protein and its components. (a) SoxAX-CT1020 protein. (b) Purified complex obtained by gel permeation chromatography from the mixture containing rSoxA, rSoxX, and rCT1020. (c) Purified rSoxX. (d) Purified rSoxA. The concentration of each protein was 5 μM based on heme determination. Dithionite-reduced spectra (solid lines) were obtained by the addition of sodium dithionite. Oxidized spectra (dotted lines) are those of the preparations as obtained.