FIG. 2.

Effect of gerN and gerT mutations on the germination response to inosine as the sole germinant, measured by fall in OD of spore suspensions. Spore suspensions were incubated in 10 mM Tris-HCl, pH 8.0, 10 mM NaCl at 37°C over a range of inosine concentrations. (A) Strain B. cereus ATCC 10876 (ger⁺); (B) AM1631 (gerT1::pSMUT); (C) AM1421 (gerN17::pMUTIN4); (D) AM1632 (gerN17::pMUTIN4 gerT1::pSMUT). Symbols represent different inosine concentrations as follows: ⋄, no inosine; □, 100 μM; ○, 1 mM. The data for 10 μM inosine are superimposed on the “no inosine” line.