FIG. 2.

Gene expression of R. typhi extracytoplasmic proteins in HeLa cells. HeLa cells were infected with R. typhi at an MOI of ∼10, and the bacteria were isolated for RNA extraction at 4 days postinfection. Two-step, real-time RT-qPCR was used to assess gene expression of the putative R. typhi extracytoplasmic proteins relative to the rpsL gene. The mean relative expression and the standard errors are presented for each gene; results are from three independent infections. Individual data points are listed in Table S5 in the supplemental material.