TABLE 1.
Strains used in this study
| Strain | Nomenclature | Genotype | Source or reference |
|---|---|---|---|
| GM1596 | Wild type | trpC2 | Lab stock (168CA) |
| GM1560 | ccpN* | trpC2 ΔccpN (nonpolar) amy::PgapB′-lacZ(cat) | PS1722; Servant et al. (37) |
| GM1599 | ccpN | trpC2 Δ(ccpN-yqfL)::phleo | PS1679; Servant et al. (37) |
| GM2607 | ccpN gapB | trpC2 Δ(ccpN-yqfL)::phleo ΔgapB::pMUTIN2(ery) | GM1599 × GM1500 DNA |
| GM2611 | ccpN gapB pckA | trpC2 Δ(ccpN-yqfL)::phleo ΔgapB::pMUTIN2(ery) ΔpckA::spec | GM2607 × GM2664 DNA |
| GM2609 | ccpN pckA | trpC2 Δ(ccpN-yqfL)::phleo ΔpckA::spec | GM1599 × GM2664 DNA |
| GM2664 | pckA | trpC2 ΔpckA::spec | Lab stock |
| GM2677 | sr1 | trpC2 Δsr1::cat | GM1596 × pINT1 |
| GM2678 | ccpN sr1 | trpC2 Δ(ccpN-yqfL)::phleo Δsr1::cat | GM1599 × GM2677 DNA |
| GM2690 | ccpN gapB | trpC2 Δ(ccpN-yqfL)::phleo ΔgapB::pEC23(kan) | GM1599 × GM2648 DNA |
| GM1500 | gapB | trpC2 ΔgapB::pMUTIN2(ery) | Fillinger et al. (11) |
| GM2648 | gapB | trpC2 ΔgapB::pEC23(kan) | GM1500 × pEC23 |
| GM2691 | ccpN gapB pckA | trpC2 Δ(ccpN-yqfL)::phleo ΔgapB::pEC23(kan) ΔpckA::spec | GM2611 × GM2648 DNA |
| GM1501 | gapA | trpC2 ΔgapA::pMUTIN2(ery) | Fillinger et al. (11) |
| GM2676 | ccpN gapA | trpC2 Δ(ccpN-yqfL)::phleo ΔgapA::pMUTIN2(ery) | GM1599 × GM1501 DNA |
| GM2679 | ccpN gapA sr1 | trpC2 Δ(ccpN-yqfL)::phleo ΔgapA::pMUTIN2(ery) Δsr1::cat | GM2678 × GM2676 DNA |
| GM2705 | pckA | trpC2 ΔpckA::neo | Lab stock |
| GM2721 | amyE::Phyp | trpC2 amyE::Phyperspank/lacI (spec) | GM1596 × pDR111 |
| GM2723 | amyE::Phyp-gapB | trpC2 amyE::Phyperspank-gapB/lacI (spec) | GM1596 × pIC586 |
| GM2725 | amyE::PhyppckA | trpC2 amyE::Phyperspank/lacI (spec) ΔpckA::neo | GM2705 × GM2721 DNA |
| GM2727 | amyE::Phyp-gapB pckA | trpC2 amyE::Phyperspank-gapB/lacI (spec) ΔpckA::neo | GM2705 × GM2723 DNA |
| GM2730 | ccpN sr1 gapB pckA | trpC2 Δ(ccpN-yqfL)::phleo ΔgapB::pEC23(kan) ΔpckA::spec Δsr1::cat | GM2691 × GM2677 DNA |
| GM2739 | sr1 pckA | trpC2 ΔpckA::spec Δsr1::cat | GM2664 × GM2677 DNA |
| GM2740 | sr1 gapB pckA | trpC2 ΔpckA::spec Δsr1::cat ΔgapB::pEC23(kan) | GM2739 × GM2648 DNA |