Fluid accumulation of rabbit intestinal loops treated with different doses of purified CPB. Rabbit duodenal (top), jejunal (middle), or ileal (bottom) loops were treated with Ringer's solution containing 1 (CPB 1), 5 (CPB 5), 10 (CPB 10), or 20 (CPB 20) μg of purified CPB and 150 μg of TI/ml; 20 μg of purified CPB with no TI (CPB20/NTI); or Ringer's solution and 150 μg of TI/ml (control). After a 6-h treatment, fluid accumulation was recorded as described in Materials and Methods. Fluid accumulation in all loops injected with any dose of CPB plus TI was statistically different from control loops (*, P > 0.05) or loops treated with CPB and no TI (**, P > 0.05). Every experiment was independently performed six times; the data shown represent the mean of these studies, and small bars represent standard errors.