FIG. 3.

rAls3p-N vaccine induced a moderated type 1 immune response during murine staphylococcemia. Mice were vaccinated with CFA alone (n = 5) or CFA plus rAls3p-N (n = 7) and infected i.v. with S. aureus 67-0 (2 × 10⁷ organisms). Organs were harvested on day 5 postinfection. Frequencies of Th1 (CD4⁺ IFN-γ⁺ IL-4⁻) or Th2 (CD4⁺ IFN-γ⁻ IL-4⁺) splenocytes or Th1/Th2 ratios were determined by intracellular cytokine staining and flow cytometry. Bars reflect median values. *, P < 0.05 compared to results for CFA alone by the Mann-Whitney U test.