FIG. 5.
rAls3p-N vaccine administered with Al(OH)3 adjuvant primes murine lymphocytes for both Th1 and Th2 responses. Two weeks following the booster dose, splenocytes were cultured from BALB/c mice (n = 10 per group) vaccinated with rAls3p-N (300 μg) plus Al(OH)3 or Al(OH)3 alone. Cells were stimulated ex vivo with rAls3p-N (100 μg/ml). (A) Proliferation of splenic lymphocytes from vaccinated or control mice after 3 days of coculture with rAls3p-N. (B) Frequency of Th1 (CD4+ IFN-γ+) or Th2 (CD4+ IL-4+) splenocytes after 4 days of coculture with rAls3p-N, as determined by intracellular cytokine analysis. (C) Cytokine content in supernatants after 4 days of splenocyte coculture with rAls3p-N. Median and interquartile ranges are shown. *, P < 0.05 compared to results for alum-administered control mice by the Mann-Whitney U test.