TABLE 1.
C. jejuni strains and their invasiveness for Caco-2 cells
| Straina | LOS locus | % Invasionb | No. of invading C. jejuni organisms per 100 cells | Presence of NeuAcc | Ganglioside mimicd | Illness |
|---|---|---|---|---|---|---|
| GB2 | A | 3.4 ± 0.55 | 285-395 | Yes | GM1a, GD1a | GBS |
| GB11 | A | 2.2 ± 0.7 | 150-290 | Yes | GM1a, GD1a | GBS |
| GB19 | A | 0.8 ± 0.29 | 51-109 | Yes | GD1c | GBS |
| GB3 | A | 0.12 ± 0.046 | 7-16 | Yes | GM1a, GD1a | GBS |
| GB22 | A | 0.05 ± 0.026 | 3-7 | Yes | GM1a, GD1a | GBS |
| GB23 | A | 1.17 ± 0.14 | 103-131 | Yes | GM2 | GBS |
| GB29 | A | 0.73 ± 0.06 | 67-79 | GBS | ||
| E990521 | A | 3.0 ± 1.15 | 185-415 | Enteritis | ||
| E991095 | A | 1.9 ± 0.81 | 110-271 | Enteritis | ||
| E9126 | A | 1.2 ± 0.58 | 70-178 | Enteritis | ||
| P19 | A | 4.7 ± 1.4 | 330-610 | Yes | GM1a, GD1a | Enteritis |
| P10 | A | 4.23 ± 1.86 | 237-609 | Yes | GD3 | Enteritis |
| P4 | A | 0.0054 ± 0.00092 | 0.44-0.63 | Yes | GM1a, GD1a | Enteritis |
| GB17 | B | 3.05 ± 1.75 | 130-480 | Yes | GM1b, GD1c | GBS |
| GB25 | B | 0.27 ± 0.13 | 14-40 | Yes | GM1b, GD1c | GBS |
| GB31 | B | 0.97 ± 0.15 | 82-112 | Yes | GM1a, GD1a | GBS |
| GB37 | B | 0.16 ± 0.03 | 13-19 | GBS | ||
| Rivm 16 | B | 1.98 ± 0.7 | 192-205 | Enteritis | ||
| Rivm 38 | B | 0.037 ± 0.023 | 1.0-6.0 | Enteritis | ||
| Rivm 129 | B | 0.084 ± 0.026 | 5.0-11 | Enteritis | ||
| E989123 | B | 0.29 ± 0.011 | 18-40 | Enteritis | ||
| E981033 | B | 0.26 ± 0.075 | 18-33 | Yes | GM1a | Enteritis |
| E98652 | B | 0.028 ± 0.006 | 2-4 | Yes | GM1a, GQ1b | Enteritis |
| 81-176 | B | 0.26 ± 0.06 | 20-32 | Yes | GM2, GM3 | Enteritis |
| GB13 | C | 0.2 ± 0.017 | 18-22 | Yes | GM1a | GBS |
| GB38 | C | 1.8 ± 0.77 | 103-257 | GBS | ||
| Rivm 15 | C | 0.00075 ± 0.00014 | 0.061-0.089 | Enteritis | ||
| Rivm 83 | C | 2.75 ± 1.28 | 147-403 | Enteritis | ||
| Rivm 93 | C | 3.5 ± 1.15 | 235-465 | Enteritis | ||
| Rivm 109 | C | 1.22 ± 0.44 | 78-166 | Enteritis | ||
| Rivm 116 | C | 0.25 ± 0.13 | 12-38 | Enteritis | ||
| E98682 | C | 0.010 ± 0.0036 | 0.6-1.4 | Yes | GM1a, GQ1b | Enteritis |
| E981087 | C | 0.13 ± 0.031 | 10-16 | Yes | GM1a | Enteritis |
| P1 | C | 0.01 ± 0.001 | 0.9-1.1 | Yes | GM2 | Enteritis |
| P2 | C | 0.005 ± 0.0017 | 0.33-0.67 | Yes | GM1b | Enteritis |
| Rivm 3 | D | 0.005 ± 0.0012 | 0.38-0.62 | Enteritis | ||
| Rivm 33 | D | 0.017 ± 0.0045 | 1-2 | Enteritis | ||
| Rivm 65 | D | 0.018 ± 0.0026 | 1-2 | Enteritis | ||
| Rivm 67 | D | 0.0097 ± 0.0013 | 0.5-1 | Enteritis | ||
| Rivm 95 | D | 0.019 ± 0.003 | 1-2 | Enteritis | ||
| Rivm 104 | D | 0.0082 ± 0.0014 | 0.68-0.96 | Enteritis | ||
| E98706 | D | 0.014 ± 0.0025 | 1.15-1.65 | No | None | Enteritis |
| E970873 | D | 0.14 ± 0.02 | 12-16 | Enteritis | ||
| GB4 | E | 0.009 ± 0.003 | 0.5-1 | No | None | GBS |
| Rivm 37 | E | 0.081 ± 0.029 | 5-11 | Enteritis | ||
| Rivm 46 | E | 0.0065 ± 0.0027 | 0.38-0.92 | |||
| Rivm 47 | E | 0.097 ± 0.028 | 6-12 | Enteritis | ||
| Rivm 50 | E | 0.0065 ± 0.00096 | 0.56-0.74 | Enteritis | ||
| Rivm 61 | E | 0.011 ± 0.0066 | 1-2 | Enteritis | ||
| E9141 | E | 0.074 ± 0.013 | 5-9 | Enteritis | ||
| E9144 | E | 0.14 ± 0.03 | 11-17 | Enteritis | ||
| E9146 | E | 0.08 ± 0.015 | 6-10 | Enteritis | ||
| E98623 | E | 0.004 ± 0.0015 | 0.2-0.5 | No | None | Enteritis |
| E98624 | E | 0.003 ± 0.00075 | 0.23-0.4 | No | None | Enteritis |
| P3 | E | 0.0045 ± 0.0013 | 0.32-0.58 | No | None | Enteritis |
GB, GBS-associated strain; E, enteritis-related strain; P, Penner serotype strain. Strain 81-176 was used as a positive control.
Data are means ± standard deviations for at least three independent experiments and are calculated as the percentage of bacteria that survived the gentamicin treatment.
Determined by mass spectrometry or immunological methods for the 25 strains for which results are shown. Data were not available for the other strains.
The LOS structures showing the ganglioside mimics of 18 strains were elucidated by mass spectrometry and immunological methods; for 7 strains, LOS structures were elucidated by immunological methods only. Data were not available for the other strains.