. 2008 Aug 11;76(10):4385–4395. doi: 10.1128/IAI.00394-08

TABLE 2.

Sequences of the primers and probes used for TaqMan qRT-PCR

Gene target	Sequence (5′→3′)		Amplicon size (bp)
Gene target	Primer	Probe	Amplicon size (bp)
TLR1	CATTCCGCAGTACTCCATTCAGCCCATGTTTGCTCTTTTC	CAAGCTCAAAAATCTCATGGCCAGGA^a	102
TLR2	TGATGCTGCCATTCTTGTTCGCCACTCCAGGTAGGTCTTG	CGCTTCTGCAAGCTGCGGAAGATAAT^a	107
TLR4	GAGAACTTCCCCATTGGACACTGGAAAGGTCCAAGTGCTC	TGTGGCTCACAATCTTATCCAGTCT^a	128
TLR5	GCCAGTCCTGTGTTTGGATTCTCAGCAGGAGCCTCTCAGT	TGCTCCTTTGATGGCCGAATAGCCT^a	119
GAPDH	CTGCACCACCAACTGCTTAGGATGGCATGGACTGTGGTC	CTGGCCAAGGTCATCCATGACAACT^b	91

Labeled at the 5′ end with 6-carboxyfluorescein and terminally quenched at the 3′ end with Black Hole Quencher-1.

Labeled at the 5′ end with HEX (6-carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein) and terminally quenched at the 3′ end with Black Hole Quencher-1.