FIG. 1.

Expression of RIVET fusions in vitro. RIVET fusion strains ALZ18, ALZ16, and ALZ17, for hyb, hyd, and hya, respectively, were grown overnight under conditions previously shown to induce the promoter or under noninducing conditions (aerobically [aer] or anaerobically [ana] on CR-Hyd, LB, or BA medium). β-Galactosidase activities were measured (A), and tetracycline sensitivity was determined (B) (n = 3 to 7). According to the Mann-Whitney-Wilcoxon test, β-galactosidase activities were significantly higher for ALZ17 cells grown anaerobically on BA than for cells grown aerobically on LB agar (α = 0.01).