FIG. 3.
Expression of hyd genes in RAW 264.7 murine macrophages. RAW 264.7 murine macrophages (1 × 105) were infected with 1 × 106 CFU of RIVET fusion strain ALZ16 (hyd) at an MOI of 10 in 24-well plates. Tissue culture cells were lysed with Triton X-100 at various time points, as indicated, and bacterial cells were diluted in PBS and plated. Hydrogenase expression was monitored by determining the ratio of tetracycline-sensitive colonies to ampicillin-resistant colonies, as described in Materials and Methods. Results are averages for four replicates.