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Hydrogenase mutant survival in RAW 264.7 murine macrophages. RAW 264.7 murine macrophages (1 × 105) were infected at an MOI of 10 (1 × 106) with wild-type (wt) or hyb, hyd, or hya mutant cells (strain ALZ7, ALZ8, or ALZ9, respectively). Extracellular bacteria were killed with 100 μg/ml gentamicin at 2 h p.i. Macrophages were lysed with 1% Triton X-100 at various time points, and bacteria were enumerated by dilution plating on LB. Results are averages for four replicates.
