FIG. 3.

Effects of EDDHA on extracellular proteins from stationary-phase P. aeruginosa PAO1. Extracellular proteins were prepared from bacteria grown in LB broth containing EDDHA and separated on 2-DE gels (pH gradient for isoelectric focusing, pH 4 to 7). Shown are proteins detected by Coomassie staining. The numbers represent the protein spots listed in the text. The letters represent proteins that were observed only following the addition of EDDHA to the growth medium. The approximate positions of molecular mass markers (kDa) are indicated.