FIG. 4.

Extracellular proteins from P. aeruginosa strain Pa4. Extracellular proteins were prepared from bacteria grown in LB broth until stationary phase and separated on 2-DE gels (pH gradient for isoelectric focusing, pH 4 to 7). (A) Proteins detected by Coomassie staining. (B to D) Proteins detected by sera from CF patients (2, 3, and 4) following Western blotting. The numbers represent the protein spots listed in the text. The letters represent proteins produced by strain PA4 but not observed for PAO1. The approximate positions of molecular mass markers (kDa) are indicated. Western blots with patient 1 serum were excluded due to high background.