TABLE 1.

Protein identities determined for PAO1 proteins^a

Spot no.b	PA no.c	Protein	Functiond	No. of matching peptides	Sequence coverage (%)	pIe	Mass (kDa)e	Immunoblotf
								1	2	3	4
Logarithmic-phase proteins
1	PA1092	FliC	Flagellin type B	19	43.6	5.4	49.2	+	+	+	+
2	PA1092	FliC	Flagellin type B	18	43.6	5.4	49.2	+	+	+	+
5	PA1074	BraC	Amino acid binding protein BraC	17	51.6	5.16	36.9	+	+	+	+
7	PA2951	EtfA	Electron transfer flavoprotein, α subunit	9	45.3	4.98	31	+	+	+	+
8	PA0974	PA0974	Unknown	12	40.9	7.80	29	+	+	−	+
11	PA0277	SpeB	Agmatine ureohydrolase	9	30.2	6.08	26.6	+	+	+	+
20	PA0634	PA0634	Unknown	10	50.4	5.06	12.4	−	+	+	+
21	PA0624	PA0624	Unknown	6	68	4.62	12	+	+	+	+
23	PA4385	GroEL	Chaperone GroEL	22	41.5	5.04	57.1	+	+	+	+
Stationary-phase proteins
11	PA0423	PasP	Protease	10	67.5	6.1	20.8	+	+	+	+
28	PA2939	PA2939	Aminopeptidase	6	9.9	5.1	57.5	−	+	−	+
57	PA4922	Azu	Azurin	2	23.4	6.9	16.0	+	−	−	+
72	PA1178	OprH	N-terminal fragment (10 kDa) of porin OprH	7	38.8	9.0	21.6	+	+	+	+

^aProteins were separated by 2-DE, and their identities were determined by peptide mass mapping.

^bSpot numbers are shown in Fig. 1 (exponential-phase proteins) and Fig. 2 (stationary-phase proteins).

^cPA no. is the open reading frame number in the P. aeruginosa strain PAO1 genome (http://www.pseudomonas.com).

^dProtein functions were predicted through BLASTP and TBLASTN searches, with at least 50% protein sequence identity obtained in each case.

^eValues for pI and mass are theoretical values from deduced amino acid sequence of the identified gene and open reading frame, respectively.

^fImmunoblot refers to detection (+) or no detection (−) by sera from patients 1, 2, 3, and 4 (Fig. 1 and 2).