FIG. 5.

Topical application of MAb F429 onto the eyes of mice starting 8 h after infection and repeated at 24 and 32 h after infection reduces bacterial levels and associated corneal pathology. Both extracellular and internalized CFU per eye were reduced after challenge with two of three invasive strains (PAO1 and 6294 but not 6354) or all three cytotoxic strains (PAO1 ExoU⁺, 6077, and 6206), as was corneal pathology. Points indicate values for an individual mouse, bars indicate medians, and P values were calculated by the Mann-Whitney U test. Infectious inocula (CFU per eye given in a 5-μl volume): PAO1, 10⁶; 6294, 2.2 × 10⁶; 6354, 10⁶; PAO1 ExoU⁺, 9 × 10⁵; 6077, 8.5 × 10⁵; 6206, 8.5 × 10⁵.