Skip to main content
. 2008 Jul 21;76(10):4783–4791. doi: 10.1128/IAI.01612-07

FIG. 3.

FIG. 3.

Caspase activities in CDT-treated MOLT-4 cells in the presence of z-VAD-fmk. Caspase activities were monitored in MOLT-4 cells at 24 or 48 h after CDT treatment (100 ng/ml) in the presence or absence of z-VAD-fmk (100 μM). CDT-treated cell extracts (5 μg of total protein) were incubated with 50 μM concentrations of the various fluorigenic substrate peptides: AMC for caspase-3, caspase-7, and caspase-8; Ac-DQTD-AMC for caspase-7 and caspase-3; Ac-IETD-AMC for caspase-8, caspase-6, and Granzyme; Ac-LEHD-AMC for caspase-9; and Ac-VDVAD-AMC for caspase-2 (Peptide Institute, Inc., Osaka, Japan). The reaction mixture was incubated at 37°C for 60 min, where the release of AMC was measured by using a fluorometer (Shimazu RF-540) with an excitation at 380 nm and an emission at 460 nm.