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Growth in BHI triggers host adaptation of human-virulent F. tularensis. Lysates (10 μg/lane) of F. tularensis SchuS4 grown to late log phase in MHB and BHI were resolved by SDS-PAGE and stained with Coomassie blue (A) or transferred to nitrocellulose for Western blotting (B). The arrowheads in panel A and numerical values in panel B are as described in the legend to Fig. 1.
