FIG. 4.
Analysis of airway cytokine/chemokine expression in C57BL/6J and SJL/J mice at 7 and 14 days after C. neoformans lung infection. The levels of TNF-α (A), KC/CXCL1 (B), IL-6 (C), IFN-γ (D), IL-12/IL-23p40 (E), and IL-4 (F) were measured in the BALF of C57BL6J and SJL/J mice that had been infected with a serotype D C. neoformans isolate for 7 or 14 days. Each bar represents the mean ± standard error of the mean (n ≥ 5 mice/group/time point; *, P < 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ND, not detected). (G) RT-PCR analysis of lung IFN-γ mRNA expression in C57BL/6J and SJL/J mice at 7 days after infection with a serotype D C. neoformans isolate. Amplification of mouse β-actin was performed as a control.