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Quantitative real-time expression of eefX, eefA, and eefB in the ΔeefA and the cis-complemented (cisΔeefXA) mutants compared to their expression in wild-type strain LM21. The expression of eefX, eefA, and eefB was measured by the ΔΔCT method, in which the 16S rRNA was used as an internal reference and wild-type cells were used as the calibrator. Levels of change (n-fold) in eefX, eefA, and eefB with respect to the wild type are shown for the ΔeefA and the cis-complemented mutants.
