TABLE 1.
Bacterial strains and plasmids used in this study
| Plasmid or strain | Description | Source or reference |
|---|---|---|
| Klebsiella pneumoniae | ||
| LM21 | Wild-type strain; Apr | 9 |
| C-81 | LM21 with mini-Tn5 inserted in eefA; Apr, Kmr | 17 |
| LM21Spt | LM21 ΔSHV1::aadA7; Spr | This study |
| LM21ΔeefA | LM21 ΔSHV1::aadA7 ΔeefA::aph3; Spr, Kmr | This study |
| LM21ΔeefAApr | LM21 ΔeefA::aph3; Kmr, Apr, Spr (spontaneous mutant) | This study |
| LM21ciseefXA | LM21 ΔSHV1::Φ (eef-eefX-eefA-aadA7) ΔeefA::aph3; Spr, Kmr | This study |
| Φ (eef-lacZ) | LM21 ΔeefA::lacZ-aph3; Kmr | This study |
| LM21CH199 | LM21 containing the pKOBEG199 plasmid; Apr, Tcr | |
| Escherichia coli K-12 | ||
| JM109 | F′ traD36 proA+B+lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14−gyrA96 recA1 relA1 endA1 thi hsdR17 | |
| C600 | F−thr-1 leuB6 thi-1 lacY1 glnV44 rfbD1 fhuA21 | 27 |
| CH158 | Pop3ybeW::aph3′ | 5 |
| Plasmid | ||
| pKOBEG199 | Plasmid with λ red genes under the control of the arabinose-inducible promoter | 2 |