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In vitro stability of pGEN222 and pGEN222I plasmids in CVD 908-htrA and CVD 1208S live vectors. Live vector strains were passaged at 37°C from an overnight starter culture (grown without selection) every 24 h for 96 h in antibiotic-free liquid medium. Plasmid stability is reported as the percentage of fluorescing colonies from the total number of CFU plated on nonselective medium.
