TABLE 1.

Primers used in the construction and testing of mchI expression plasmids

Primer	Sequencea	Cassette created	Templateb	Reference or source
1	5′-GCTAGCGGGCACCCCAGGCTTTCCACTTTATGCTTCCGGCTCGTATAATGTGTGGAATCT-3′	lacUV5 promoter variant 33C	NA	18
2	5′-TAACTCATTTTTTTTTCCTCCTTATTTTCTAGATTCCACACATTATACGAGCCGGAAGCA-3′	lacUV5 promoter variant 33C	NA	18
3	5′-TCTAGAAAATAAGGAGGAAAAAAAAATGAGTTATAAAAAACTGTACCAATTGACGGCTATAT-3′	mchI	RYC1000(pEX4)	37
4	5′-GCTAGCCTATTAACTTCCGTTTTTCCAGCCTGACCAGACCAAAATCTTTCGAATCCCATGA-3′	mchI	RYC1000(pEX4)	37
5	5′-GTAAACTTGGTCTGACAGGTACCAATGCTTAATCAGTG-3′		pGENMch	This study
6	5′-CACTGATTAAGCATTGGTACCTGTCAGACCAAGTTTAC-3′		pGENMch	This study
7	5′-CAAATAGGGGTACCGCGCACATTTCCCCG-3′		pGENMch	This study
8	5′-CGGGGAAATGTGCGCGGTACCCCTATTTG-3′		pGENMch	This study

^aRelevant restriction sites are designated in boldface (see the text for details); ribosome binding sites and start codons are in italics.

^bNA, not applicable.