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IL-8 (A) and TNF-α (B) release by CBMC is dependent on S. aureus internalization. CBMC were left uninfected, infected with S. aureus at 37°C for 180 min in the presence or absence of cytochalasin D, or incubated with PGN (10 μg m−1) at 37°C for 180 min in the presence or absence of cytochalasin D. Supernatants were analyzed for IL-8 and TNF-α. The values are the means ± standard deviations from three independent experiments (*, P < 0.01; **, P < 0.001).
