TABLE 3.

Production of MSH and MSH intermediates by M. smegmatis mutants

Enzyme	Strain	Concn (μmol/g dry weight) of MSH or intermediate
		GlcNAc-Ins	GlcN-Ins	Cys-GlcN-Ins	MSH
Wild typea	mc2155	<0.1	0.2-1.0	∼0.008	10 ± 3
MshAb	mshA::Tn5	<0.01	<0.01	<0.01	<0.01
MshBc	Myco504	2.6 ± 0.2	<0.01	<0.02	1.0 ± 0.2
MshCd	Tn1	NDe	2.6	<0.002	<0.004
MshDf	mshD::Tn5	ND	0.35 ± 0.05	0.6-2	0.12 ± 0.01g

^aFrom references 1, 59, and 94.

^bFrom reference 91.

^cFrom reference 117 and G. L. Newton, unpublished data.

^dFrom reference 118.

^eND, not determined.

^fFrom references 59 and 94.

^gTwo to 3 μmol/g of formyl-Cys-GlcN-Ins and 0.12 ± 0.01 μmol/g of succinyl-Cys-GlcN-Ins were also produced.