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. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: Biochemistry. 2006 Oct 24;45(42):12853–12858. doi: 10.1021/bi0613224

Figure 2.

Figure 2

Fluorescence titration of pyrene actin by S1 in 50 nM pyrene actin plus 2 μM native (dots) and ASα- ASβTm (dashes) titrated with S1 between 0 and 250 nM. The data was fitted to the 2-state cooperative binding model with the fits superimposed as a solid line on the raw data. Fitted parameters are given in Table 1. Conditions: 100 mM KCl, 5 mM MgCl2, 20 mM MOPS, pH 7.0, 20° C.