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. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: Biochemistry. 2005 Sep 20;44(37):12355–12361. doi: 10.1021/bi050620i

Figure 2.

Figure 2

Basal shedding of syndecan-1 ectodomain is reduced in mutants with domain swap containing amino acid residues A243SQSL247. CHO cells were transfected with wild type syndecan-1cDNA, syndecan-1CD cDNA or syndecan-1 mutant cDNAs, syn-1D, syn-1E, and syn-1F (cf Figure 1). Basal shedding was analyzed by incubating the transfected cells in 6 well plates in serum-free medium for 15 minutes. The soluble syndecan-1 ectodomain in the conditioned media was analyzed by a dot blot assay and normalized to the syndecan-1 content in total cell lysates. Expression of syndecan-1 in the total cell lysate was extracted was determined to be similar among all the cDNA constructs (data not shown). The relative shedding rate is expressed as the percentage of control (wild-type Syn-1) value. (Error bars = SD, n=3).