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. Author manuscript; available in PMC: 2008 Dec 11.
Published in final edited form as: Biochemistry. 2007 Nov 13;46(49):14153–14161. doi: 10.1021/bi701151t

Figure 3. Substrate-Dependence of MsrBA Mediated Repair of CaMox.

Figure 3

MsrBA-dependent rates of repair of CaMox as a function of NADPH (A), thioredoxin (B), or thioredoxin reductase (C). Experimental conditions included MsrBA (0.5 μM) and CaMox (30 μM) in 10 mM Tris-HCl (pH 7.5) containing indicated substrate concentrations and saturating concentrations of NADPH (400 μM) (B and C), thioredoxin (50 μM) (A and C) or thioredoxin reductase (2 μM) (A and B) at 24 °C. Line in panel B represents nonlinear least-squares fit to the Michaelis-Menten rate equation, where KM = 3.3 ± 0.8 μM.