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. 2008 Jul 9;82(18):9043–9055. doi: 10.1128/JVI.00925-08

TABLE 2.

Summary of E1b55K mutant phenotypesa

Protein Substrate degradationb phenotype (during coexpression with E4orf6 alone) for:
Substrate degradationc phenotype (during virus infection) for:
Substrate bindingd phenotype for:
Aggregate colocalizatione phenotype for:
Cytoaggregatef phenotype Nuclear retention by E4orf6g Concatemer formationh phenotype Additional reference(s)j
p53 MRN Lig4 p53 MRN Lig4 p53 MRN p53 MRN
WT E1b55K + + + + + + + + + + + + −
L83,87,89A (NES mutant protein) ND + ND ± + ND + + + + NA + ND 20, 31, 36, 39
K104R ND + ND + + ND ND + ND + + + ND 21, 36
H224 insertion mutant protein + + + + + + + + + ± + ± − 26, 34, 52, 72
R240A − + + − + + − + − ± + + − 12, 56
C348,351S ND + ND ND ND ND ND ND ND ND + + ND
H354 insertion mutant protein + − + + − + + − + − + + − 12, 26, 34, 42, 52, 71, 72
C361,366S − − − − − − ± − + − + ± +
H373A + − + + − + + − + − + + −
R443 insertion mutant protein ± − + ± − ± + − + − +/diffuse ± + 26, 34, 52, 58, 71, 72
Y444A + + + + ± + + ± + ± + ± − 56
C454,456S − − − − − − + − + −i + ± + 31
S490,491,T495A (3XPA) − + ± − − − − − NA NA Diffuse ± + 50, 62
S490,491,T495D (3XPD) ± + + ND ND ND + + + ± + + −
a

Unless otherwise indicated, symbols and abbreviations are as follows: +, proficient (may not reflect WT E1b55K activity); ±, defective; −, very defective; ND, not determined; NA, not applicable.

b

Degradation of endogenous proteins by E1b55K mutant forms in the presence of rAdE4orf6 as assessed by Western blotting or immunofluorescence analysis.

c

Degradation of endogenous proteins assessed in the context of viruses expressing E1b55K mutant proteins or E1b55K mutant cell lines infected with dl3112.

d

Interaction measured by coimmunoprecipitation experiments.

e

MRN or p53 colocalization with E1b55K aggregates as assessed by immunofluorescence.

f

E1b55K staining in the absence of rAdE4orf6 was assayed by using immunofluorescence. +, presence of E1b55K aggregates; diffuse, diffuse cytoplasmic E1b55K. See Fig. 4A and the corresponding legend for data and details.

g

E1b55K staining in the presence of rAdE4orf6 was assayed by using immunofluorescence. See Fig. 4A and the corresponding legend for data and details.

h

Concatemer formation by mutant virus dl3112 measured in mutant E1b55K-expressing cells.

i

Observation from the present study based on E1b55K expression in human cells.

j

This table summarizes data from this paper as well as the additional references indicated.