FIG. 5.

TNTase activity under RNA replication conditions. 26n_RNA (left panel) and 6n_RNA (right panel) oligonucleotides were incubated with the P2 polymerase in the presence of different additives as indicated below the panel. Shown is an autoradiogram of the reaction products after separation on a denaturing 20% polyacrylamide gel. The RNA or the donor UTP was radioactively labeled (marked with an asterisk). The mobilities of RNA molecules of different sizes are indicated on the left sides of the panels (based on the single nucleotide ladder defined in the legend to Fig. 2). The reaction products within each lane (1 to 4) are schematically depicted below the panels. A black line represents the prelabeled RNA oligonucleotide, a gray line an unlabeled RNA, a black asterisk a labeled UMP, and a gray asterisk an unlabeled UMP incorporated into the RNA.