Knockdown of class III PI3K components reduces CVB3 replication. (A) Western blot analysis of VP1 and Beclin-1 or VPS34 expression in CVB3-infected cells transfected with Beclin-1 (left) or VPS34 (right) siRNA prior to CVB3 infection. VP1 expression was quantitated, normalized, and analyzed as described in the legend to Fig. 3 (data are means ± SE; n = 3). *, P < 0.05 compared to scramble siRNA control. (B) Plaque assay results for scramble or Beclin-1 and VPS34 siRNA-transfected cells (means ± SE; n = 3). *, P < 0.05; #, P < 0.001 (compared to scramble siRNA-transfected cells).