FIG. 4.

Analysis of FMDV movement through early endosomes. COS-1 cells were infected with either type O₁Campos (a to b) or type O₁C3056R-KGE (c to d) at 4°C as described in Materials and Methods. MCF-10A cells were infected with either type O₁Campos (e to g) or type O₁C3056R-KGE (h to j) at 4°C as described in Materials and Methods. Cells were shifted to 37°C and, at the times indicated over each panel, were fixed and probed, as described in Materials and Methods, for viral proteins (red), EEA-1 (green), or TfnR (green). Only the merged photomicrographs are shown.