FIG. 1.

NY-1V Gn cytoplasmic tail inhibits TBK1- and TRAF2-directed NF-κB activation. HEK 293 cells were transfected with 250 ng ISRE promoter (A) or NF-κB luciferase reporters (B and C) with or without TBK1 (500 ng) or TRAF2 (500 ng) expression vector. Where indicated, cells were transfected with increasing amounts of NY-1V or PHV Gn cytoplasmic tail expression vectors (1 to 2 μg), NY-1V nucleocapsid protein expression vector (2 μg), or control vector in order to transfect cells with a constant amount of DNA. Luciferase reporter activity was assayed 48 h posttransfection, was normalized to Renilla luciferase levels, and is reported as the increase over controls lacking TBK1 or TRAF2 activation.