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. 2008 Jul 9;82(18):9115–9122. doi: 10.1128/JVI.00290-08

FIG. 3.

FIG. 3.

The NY-1V Gn cytoplasmic tail binds the N-terminal domain of TRAF3. HEK 293 cells were cotransfected with GAL4, NY-1V Gn, or PHV Gn expression plasmids along with FLAG-TRAF3 (N415). Transfected cells were treated with MG132 (50 μM) for 6 h prior to cell lysis and analysis. Two days posttransfection and following anti-GAL4 antibody immunoprecipitation (IP), NY-1V and PHV Gn tail expression was analyzed by anti-GAL4 Western blotting (center panel). Immunocomplexes were isolated on protein A/G agarose beads, and coimmunoprecipitated TRAF3 (N415) protein was analyzed by anti-FLAG Western blotting (WB) (top panel). TRAF3 (N415) expression was determined by anti-FLAG Western blotting (bottom panel). IgG heavy chain (HC) and light chain (LC) are indicated.