FIG. 5.

NY-1V but not PHV infection disrupts TBK1-TRAF3 interactions. Vero E6 cells were transfected with TBK1 and TRAF3 expression plasmids, and 24 h posttransfection cells were infected with NY-1V or PHV or mock infected. One day postinfection cells were lysed and TBK1 was immunoprecipitated (IP) with anti-myc antibody. Coprecipitated TRAF3 was detected by anti-FLAG Western blotting (WB). Immunoprecipitated TBK1 and input TRAF3 were determined by Western blotting (center panels). NY-1V- and PHV-infected cells were determined by Western blotting for nucleocapsid protein (N-protein) 24 h postinfection by using an anti-N-protein rabbit polyclonal antiserum (bottom panel). HC, IgG heavy chain.