FIG. 5.

Vif-induced G₂ accumulation does not impair cell division and is relieved by caffeine. (A) SupT1 cells were stained with CFSE and then infected with the indicated DHIV vectors or treated with doxorubicin. At 24-h intervals, cells were stained with anti-Thy1-PE antibody. Cells positive for the Thy1 marker were electronically gated and evaluated for the loss of green fluorescence, as an indicator of cell division. Numbers indicate the mean fluorescence intensity for the corresponding curves. (B) SupT1 cells were infected with the indicated viral vectors in the presence or absence of 4 mM caffeine. At 48 h postinfection, the cell cycle profile was assessed.