TABLE 2.
Comparison of microarray and qRT-PCR results
| miRNA and testa | Fold change atb:
|
|||
|---|---|---|---|---|
| 6 hpi | 24 hpi | 48 hpi | 120 hpi | |
| miR-223 | ||||
| Microarray | 0 | 0 | 3 | 3 |
| qRT-PCR | 0 | 0 | 2 | 3 |
| miR-101 | ||||
| Microarray | 0 | 0 | 3 | 4 |
| qRT-PCR | 0 | 0 | 8 | 8 |
| miR-181 | ||||
| Microarray | 0 | 0 | 4 | 7 |
| qRT-PCR | 0 | 2 | 2 | 4 |
| miR-100 | ||||
| Microarray | 0 | 0 | 3 | 34 |
| qRT-PCR | 0 | 0 | 4 | 16 |
a
Short RNAs (≤200 nt) isolated from the same RNA preparations used in microarray analysis were reverse transcribed and then analyzed by qRT-PCR.
b
The change is the ratio of threshold cycles (CT), CTmock to CTinfected, for each time point. Results are shown from a subset of downregulated miRNAs.