TABLE 2.

Temperature sensitivities and neurovirulence of isolates from the immunodeficient patient

Virus	Nucleotide or amino acid position in straina:													Temp sensitivityb		Neurovirulencec (no. of paralyzed mice/total)
	5′ NCR			Capsid region						2A		3D Pol	3′ NCR
	476	480	527	VP3-1	VP1-88	VP1-90	VP1-99	VP1-101	VP1-106	2A-36	2A-134	3D-73	7441	39.5°C	40°C
Sabin 1	U	G	C	Met	Ala	Ile	Lys	Lys	Thr	Asn	Thr	His	G	>6.0	>6.0	0/8
A21	A	-	-	-	-	-	-	-	-	-	-	-	-	4.3	>6.0	1/8
A49a	A	-	-	-	-	-	-	Asn	Ala	-	Ser	-	A	1.9	4.7	5/8
A49b	A	A	-	-	-	Val	-	-	-	-	Ser	-	A	2.3	6.1	5/8
A49c	-	A	-	-	Thr	-	-	Del	Ala	-	Ser	-	A	2.1	4.6	6/8
A49d	-	A	-	-	-	-	-	-	Ala	-	Ser	-	A	2.6	5.2	4/8
A63	-	A	-	-	-	-	-	Asn	Ala	-	Ser	-	A	3.3	5.0	5/8
A84	A	A	-	Leu	-	Val	-	-	-	Asp	Ala	Tyr	A	0.9	2.6	5/8
A88	-	A	-	Leu	-	Val	Glu	-	-	Asp	Ser	Tyr	A	1.0	3.6	5/8
A91	A	A	-	Leu	-	Val	-	-	-	Asp	Ala	Tyr	A	0.6	3.5	2/8
A102	A	A	-	Leu	-	Val	Glu	-	-	Asp	Ser	Tyr	A	1.7	3.4	5/8
A105	A	A	-	Leu	-	Val	-	-	-	Asp	Ser	Tyr	A	0.4	2.8	6/8
A118	A	A	-	Leu	-	Val	-	Asn	-	Asp	Ala	Tyr	A	0.6	3.6	5/8
A199	A	A	U	Leu	-	Val	-	Asn	-	Asp	Ser	Tyr	A	0.0	0.4	7/8
A449	A	A	U	Leu	-	Val	-	Asn	Ala	Asp	Ala	Tyr	A	0.0	0.3	8/8
A649	A	A	U	Leu	-	Val	-	Asn	Ala	Asp	Ser	Tyr	A	0.1	0.6	8/8
Mahoney	U	A	C	Leu	Thr	Val	Lys	Lys	Ala	Ser	Ser	Tyr	A	0.0	0.1	6/8

^aMutations that reverted to Mahoney sequences are shown in italics, the same nucleotide or amino acid as in Sabin 1; Del, deletion.

^bVirus titer reduction in HEp-2c cells at the given temperature with respect to virus titer at 35°C expressed as log CCID₅₀.

^cNumber of mice paralyzed with a dose of virus equivalent to 6.0 log CCID₅₀ (±0.25) by the intramuscular route.